Can the tool make Toyota proximity keys virgin?

Yes if they are not 128-bit

 Is there a tool out there that can unlock 128-bit keys?

Toyota? Or Subaru. The key tool will also do Subaru as long as they are not 128-bit

I see more Toyota than Subaru keys.

TLSprog will do 128-bit Toyota as will Tango, iKeyClone, MK3, TM100, etc

(Credits to Trent Dobrzyn)